Evaluation of equilibrium constants by affinity chromatography

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Evaluation of equilibrium constants by affinity chromatography.

Theoretical expressions are derived for affinity chromatography of systems comprising an acceptor A with one binding site for attachment to a functional group X on the column matrix and one site for interaction with a small ligand B that specifically affects its elution. From a general relationship covering all possible interactions between A, B and X simpler expressions are derived for affinit...

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preparation of plasminogen by affinity chromatography

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Purification by Affinity Chromatography

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The estimation of affinity constants for the binding of model peptides to DNA by equilibrium dialysis.

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1974

ISSN: 0264-6021

DOI: 10.1042/bj1430435